ELISA: 1: 5000 - 1: 25000. Western Blot: 1: 500 - 1: 2500, detects a band of approximately 90 kDa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Target
HSP90AA1
(Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1))
EL52 antibody, HSP86 antibody, HSP89A antibody, HSP90A antibody, HSP90N antibody, HSPC1 antibody, HSPCA antibody, HSPCAL1 antibody, HSPCAL4 antibody, HSPN antibody, Hsp89 antibody, Hsp90 antibody, LAP2 antibody, Hsp86 antibody, Hspca antibody, htpG antibody, 86kDa antibody, 89kDa antibody, AL024080 antibody, AL024147 antibody, Hsp86-1 antibody, hsp4 antibody, HSP90 antibody, HSP90AA1 antibody, fb17b01 antibody, hsp90 antibody, hsp90a antibody, hsp90a.1 antibody, hsp90alpha antibody, wu:fb17b01 antibody, zgc:86652 antibody, Hsp90alpha antibody, heat shock protein 90 alpha family class A member 1 antibody, heat shock protein 90, alpha (cytosolic), class A member 1 antibody, Heat Shock Protein 90, cytosolic antibody, heat shock protein 90A antibody, molecular chaperone antibody, heat shock protein 90, alpha (cytosolic), class A member 1, tandem duplicate 1 antibody, heat shock protein HSP 90-alpha antibody, heat shock protein 90kDa alpha (cytosolic), class A member 1 antibody, HSP90AA1 antibody, Hsp90aa1 antibody, HSP90A antibody, hsp90A antibody, hsp90aa1.1 antibody, LOC108698781 antibody
Background
HSP90a is one of two HSP90 isoforms together with HSP90b. These isoforms are encoded by different genes and under normal conditions assist the folding of newly synthesised proteins, and are also important in cellular processes such as hormone signalling and cell cycle control. In response to mutation or environmental stress, HSP90 expression is upregulated as part of the heat shock response, and function to maintain newly destabilised proteins in a folding competent state. HSP90 can also affect the epigenetic state at specific loci, leading to phenotype modulation. Induction of HSP90a by c-Myc may control the activity of multiple signal pathways involved in cellular transformation.Synonyms: HSP86, HSP90A, HSPC1, HSPCA, Heat shock 86 kDa, Heat shock protein HSP 90-alpha, NY-REN-38