Aurora A antibody (pThr288)

Catalog No. ABIN319281

The Rabbit Polyclonal anti-Aurora A antibody (ABIN319281) specifically detects Aurora A in IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for Aurora A antibody (pThr288) (ABIN319281)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Aurora A Antibody

Binding Specificity: pThr288

Specificity: Aurora A (phospho-Thr288) Antibody AP08036PU detects endogenous levels of Aurora A only when phosphorylated at Threonine 288.

Purification: Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Aurora A around the phosphorylation site of Threonine 288 (R-T-TP-L-M).

Application Details

Application Notes: Immunohistochemistry (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at-20 °C.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: Aurora Kinase A

Background: Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Synonyms: AIK1, ARK1, AURA, AURKA, AURORA2, Aurora/IPL1-related kinase 1, BTAK, Breast tumor-amplified kinase, STK15, STK6, Serine/threonine kinase 15, Serine/threonine-protein kinase 6

Gene ID: 6790

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization