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HA-Tag antibody

Details for Product No. ABIN319423, Supplier: Log in to see
Antigen
Reactivity
Tag
342
16
11
2
2
2
1
1
Host
Rabbit
157
148
36
29
3
1
Clonality
Polyclonal
Conjugate
Un-conjugated
35
27
24
7
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
294
160
106
74
50
31
19
14
13
11
9
5
5
5
4
4
3
2
2
2
1
1
1
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Immunogen The antiserum was produced by immunizing rabbit with synthesized peptide containing the Influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).
Sequence YPYDVPDYA
Purification Immunoaffinity Chromatography using epitope-specific peptide.
Alternative Name HA Epitope Tag (YPYDVPDYA)
Target Type Tag
Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Application Notes Western Blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl and 50 % Glycerol, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-HA-Tag antibody (ABIN319423) anti-HA-Tag antibody
Product cited in: Qiu, Ding, Zou et al.: "Divergent roles of amino acid residues inside and outside the BB loop affect human Toll-like receptor (TLR)2/2, TLR2/1 and TLR2/6 responsiveness." in: PLoS ONE, Vol. 8, Issue 4, pp. e61508, 2013 (PubMed).

Background publications Field, Nikawa, Broek et al.: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).