HFE2 antibody (AA 234-416)
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- Target See all HFE2 Antibodies
- HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))
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Binding Specificity
- AA 234-416
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HFE2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to Hemojuvelin (HJV)
- Sequence
- MGHHHHHHSG SEF-KVYQAEV DNLPVAFEDG SINGGDRPGG SSLSIQTANP GNHVEIQAAY IGTTIIIRQT AGQLSFSIKV AEDVAMAFSA EQDLQLCVGG CPPSQRLSRS ERNRRGAITI DTARRLCKEG LPVEDAYFHS CVFDVLISGD PNFTVAAQAA LEDARAFLPD LEKLHLFPSD AGVPLSSATL LAPLLS
- Specificity
- The antibody is a mouse monoclonal antibody raised against HJV. It has been selected for its ability to recognize HJV in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Pig, Rat
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Hemojuvelin (HJV) corresdonding to Lys234~Ser416 with N-terminal His Tag
- Clone
- C5
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product HFE2 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- HFE2 (Hemochromatosis Type 2 (Juvenile) (HFE2))
- Alternative Name
- Hemojuvelin (HFE2 Products)
- Synonyms
- HFE2A antibody, HJV antibody, JH antibody, RGMC antibody, HFE2 antibody, DKFZp468F2322 antibody, 2310035L15Rik antibody, 5230400G09Rik antibody, AI414844 antibody, AI789733 antibody, DL-M antibody, Rgmc antibody, hemojuvelin antibody, RGMr antibody, hjv antibody, id:ibd3464 antibody, wu:fb38f10 antibody, zgc:136698 antibody, hemochromatosis type 2 (juvenile) antibody, hemochromatosis type 2 antibody, HFE2 antibody, Hfe2 antibody, hfe2 antibody
- Background
- HFE2, HFE2A, HJV, JH, RGMC, hemochromatosis type 2(Juvenile), Haemojuvelin, RGM domain family member C
- Pathways
- Transition Metal Ion Homeostasis
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