GZMB antibody

Catalog No. ABIN330561

The Mouse Monoclonal anti-GZMB antibody (Clone 351927) (ABIN330561) specifically detects GZMB in WB, IHC, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for GZMB antibody (ABIN330561)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 351927

Product Details anti-GZMB Antibody

Specificity: Recognizes human Granzyme B. Shows no cross-reactivity with rhGranzyme A, -H, rmGranzyme B, -C, -D, or -G.

Purification: Protein G purified

Immunogen: NS0-derived rhGranzyme B.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, Flo, IHC, WB

Usage: Western Blot, Flow Cytometry, Immunohistochemistry, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human Granzyme B. Using a colorimetric detection system, the detection limit for rhGranzyme B is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was tested for flow cytometry using NK-92 cells. For intracellular staining to detect human Granzyme B, cells must first be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin in PBS. Dilute this antibody to 25 μg/mL and add 10 μL of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to addition of a secondary developing reagent. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. Immunohistochemistry: This antibody was used at a concentration of 8-25 μg/mL with appropriate secondary reagents to detect human Granzyme B in paraformaldehyde-fixed human PBMC. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Granzyme B. The detection limit for rhGranzyme B is approximately 5 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: GZMB / Granzyme B

Background: Name/Gene ID: GZMB
Subfamily: Serine S1
Family: Protease

Synonyms: GZMB, CCPI, CGL-1, Cathepsin G-like 1, CSP-B, CTLA-1, CTLA1, CGL1, CSPB, Fragmentin 2, GRB, HLP, Human lymphocyte protein, Fragmentin-2, SECT, Lymphocyte protease, T-cell serine protease 1-3E, C11, CTSGL1, Cytotoxic serine protease B, Granzyme B, Granzyme-2

Gene ID: 3002

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis