Protein Tyrosine Phosphatase-Like N (PTPRN) antibody

Antigen: Protein Tyrosine Phosphatase-Like N (PTPRN)

Synonyms: IA2, IA-2, ICA512, R-PTP-N, FLJ16131, IA-2/PTP

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334423

Additional Information

Alternative name: IA2

Gene ID: 5798, 19275 (mouse), 116660 (rat)

Immunogen: Peptide with sequence C-QGEGNIKKNRHPD, from the internal region of the protein sequence according to NP_002837.1.

Description: All names and symbols: IA-2 , protein tyrosine phosphatase, receptor type, N , FLJ16131 , IA-2/PTP, IA2 , ICA512 , R-PTP-N, islet antigen 2 , islet cell antigen 512 , islet cell autoantigen 3 , protein tyrosine phosphatase-like N. Official Symbol: PTPRN. RefSeq number(s): NP_002837.1

Specificity: Tested: Mouse. Expected from sequence similarity: Human, Mouse, Rat.

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 110kDa band observed in lysates of cell line NIH 3T3 (calculated MW of 106kDa according to Human NP_002837.1 and Mouse NP_033011.2). Recommended concentration: 0.5-1.5µg/ml.

Concentration: 0.5 mg/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN334423 (0.5µg/ml) staining of NIH 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Buzzetti, Di Pietro, Giaccari et al.: "High titer of autoantibodies to GAD identifies a specific phenotype of adult-onset autoimmune diabetes." in: Diabetes Care, Vol. 30, Issue 4, pp. 932-8, 2007 (PubMed).