SEMA3E antibody (Internal Region)
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- Target See all SEMA3E Antibodies
- SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SEMA3E antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), ELISA
- Purpose
- Semaphorin 3E (internal)
- Sequence
- NGGRYGTTKD YPDD
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_036563.1, NP_001171600.1).
- Cross-Reactivity
- Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-NGGRYGTTKDYPDD, from the internal region of the protein sequence according to NP_036563.1, NP_001171600.1.
- Isotype
- IgG
- Top Product
- Discover our top product SEMA3E Primary Antibody
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6 μg/mL.
Western Blot: Preliminary experiments gave an approx 35 kDa band in Human Prostate lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 8
Peptide ELISA: antibody detection limit dilution 1:128000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))
- Alternative Name
- SEMA3E (SEMA3E Products)
- Synonyms
- M-SEMAH antibody, M-SemaK antibody, SEMAH antibody, coll-5 antibody, 6430702L12 antibody, AA408817 antibody, Semah antibody, mKIAA0331 antibody, semaphorin 3E antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody, SEMA3E antibody, Sema3e antibody
- Background
- SEMA3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, KIAA0331, M-SEMAH, M-SemaK, M-sema H, SEMAH, coll-5 , sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E, semaphorin 3E
- Gene ID
- 9723, 20349
- NCBI Accession
- NP_036563, NP_001171600
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