SEMA3E antibody (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E) (Internal Region)

Details for Product anti-SEMA3E Antibody No. ABIN334487, Supplier: Log in to see
Antigen
  • M-SEMAH
  • M-SemaK
  • SEMAH
  • coll-5
  • 6430702L12
  • AA408817
  • Semah
  • mKIAA0331
  • sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
  • SEMA3E
  • Sema3e
Epitope
Internal Region
24
14
12
5
5
3
3
2
1
1
1
1
1
1
Reactivity
Human
89
38
21
5
5
3
2
2
1
1
1
Host
Goat
44
37
19
Clonality
Polyclonal
Conjugate
This SEMA3E antibody is un-conjugated
7
6
5
4
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
48
41
38
17
13
11
7
3
3
2
2
2
Options
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Immunogen Peptide with sequence C-NGGRYGTTKDYPDD, from the internal region of the protein sequence according to NP_036563.1, NP_001171600.1.
Blocking Peptide Blocking peptide for this product available: ABIN369457
Specificity This antibody is expected to recognise both reported isoforms (NP_036563.1, NP_001171600.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Semaphorin 3E (SEMA3E Antibody Abstract)
Background Synonyms: SEMA3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, KIAA0331, M-SEMAH, M-SemaK, M-sema H, SEMAH, coll-5, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E, semaphorin 3E
Gene ID 9723, 20349
NCBI Accession NP_001171600, NP_036563
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 35 kDa band in Human Prostate lysates after 0.03 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2 kDa according to NP_036563.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration: 4-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Gu, Yoshida, Livet, Reimert, Mann, Merte, Henderson, Jessell, Kolodkin, Ginty: "Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins." in: Science (New York, N.Y.), Vol. 307, Issue 5707, pp. 265-8, 2005 (PubMed).

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