Cytokeratin 13 (KRT13) antibody

Antigen: Cytokeratin 13 (KRT13)

Synonyms: K13, CK13, MGC3781, MGC161462, Krt1-13, Krt-1.13

Clonality: Monoclonal (2D7)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335223

Additional Information

Alternative name: Cytokeratin 13 / Keratin K13

Swiss-Prot: P13646

Immunogen: Keratin preparation from human esophagus.

Format: Supernatant

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 2D7

Specificity: Mab 2D7 specifically recognizes keratin K13 (54 kD polypeptide, formerly designated cytokeratin 13) present in non-cornifying squamous epithelia and in transitional epithelium of the bladder. Epidermal cell culture studies have shown that keratin K13 expression is involved in either the number or the strength of desmosomal contacts between suprabasal cells of stratified squamous epithelia. Reacts with Human, most mammals incl. mouse, rat, rabbit.

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on frozen sections: 1:5 - 1:10. Immunoblotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf Life: one year from despatch.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

van Muijen, Ruiter, Franke et al.: "Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13." in: Experimental cell research, Vol. 162, Issue 1, pp. 97-113, 1986 (PubMed).

Kopan, Traska, Fuchs: "Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization." in: The Journal of cell biology, Vol. 105, Issue 1, pp. 427-40, 1987 (PubMed).