Lamin A and C antibody

Antigen: Lamin A and C

Clonality: Monoclonal (131C3)

Host: Mouse

Reactivity: Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Hamster, Dog (Canine), Sheep (Ovine)

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335390

Additional Information

Immunogen: 131C3 is a mouse monoclonal IgG1/kappa antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 131C3

Description: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

Specificity: Human, rat, mouse, bovine, hamster, dog, sheep.

Application Details

Application Notes: 131C3 reacts with an epitope located between residues 319-566 in lamin A and C. 131C3 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-Lamin A and C antibody

anti-Lamin A and C antibody (Image 2)

Publications

Publications: Pugh, Coates, Lane et al.: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997 (PubMed).

Neri, Raymond, Giordano et al.: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999 (PubMed).

Neri, Raymond, Giordano et al.: "Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions." in: Journal of cellular biochemistry, Vol. 75, Issue 1, pp. 36-45, 1999 (PubMed).