4-HNE antibody

Antigen: 4-HNE

Clonality: Monoclonal (HNEJ-2)

Host: Mouse

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN335415

Additional Information

Immunogen: 4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.

Format: Lyophilized

Isotype: IgG1 kappa

Clone: HNEJ-2

Description: During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. This antibody shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.

Specificity: Specific for 4HNE-Lysine adduct in all species. This antibody has almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.

Application Details

Application Notes: IHC, Western and/or ELISA. Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.

Purification: Ammonium sulphate purified IgG

Buffer: Lyophilized as 100 ug/mL IgG in 50mM Tris buffered saline (TBS)

Storage: Less than -20°C, stable for 5 years Recinstitute with 200µl of distilled water.

Research Area: Neurology

Restrictions: For Research Use only

Images

High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)

Publications

Tanaka, Nishiyama, Okada et al.: "Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 77, Issue 2, pp. 145-55, 1997 (PubMed).

Nakajima, Inokuchi, Shimazawa et al.: "Astaxanthin, a dietary carotenoid, protects retinal cells against oxidative stress in-vitro and in mice in-vivo." in: The Journal of pharmacy and pharmacology, Vol. 60, Issue 10, pp. 1365-74, 2008 (PubMed).

Sedlak, Saleh, Higginson et al.: "Bilirubin and glutathione have complementary antioxidant and cytoprotective roles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 13, pp. 5171-6, 2009 (PubMed).

Boretti, Buehler, DAgnillo et al.: "Sequestration of extracellular hemoglobin within a haptoglobin complex decreases its hypertensive and oxidative effects in dogs and guinea pigs." in: The Journal of clinical investigation, Vol. 119, Issue 8, pp. 2271-80, 2009 (PubMed).

Product: Toyokuni, Miyake, Hiai et al.: "The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adduct." in: FEBS letters, Vol. 359, Issue 2-3, pp. 189-91, 1995 (PubMed).

Hattori, Nishigori, Tanaka et al.: "8-hydroxy-2'-deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 733-7, 1996 (PubMed).

Kondo, Toyokuni, Iwasa et al.: "Persistent oxidative stress in human colorectal carcinoma, but not in adenoma." in: Free radical biology & medicine, Vol. 27, Issue 3-4, pp. 401-10, 1999 (PubMed).

Yamagami, Yamamoto, Kume et al.: "Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products." in: Antioxidants & redox signaling, Vol. 2, Issue 1, pp. 127-36, 2001 (PubMed).

Hung, Skepper, Burton: "In vitro ischemia-reperfusion injury in term human placenta as a model for oxidative stress in pathological pregnancies." in: The American journal of pathology, Vol. 159, Issue 3, pp. 1031-43, 2001 (PubMed).

Tanito, Nishiyama, Tanaka et al.: "Change of redox status and modulation by thiol replenishment in retinal photooxidative damage." in: Investigative ophthalmology & visual science, Vol. 43, Issue 7, pp. 2392-400, 2002 (PubMed).

Tam, Gao, Leung et al.: "Androgenic regulation of oxidative stress in the rat prostate: involvement of NAD(P)H oxidases and antioxidant defense machinery during prostatic involution and regrowth." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2513-22, 2003 (PubMed).

Yamada, Funada, Shibata et al.: "Protein-bound 4-hydroxy-2-hexenal as a marker of oxidized n-3 polyunsaturated fatty acids." in: Journal of lipid research, Vol. 45, Issue 4, pp. 626-34, 2004 (PubMed).

Liu, Ormsby, Oja-Tebbe et al.: "Gene transfer of NAD(P)H oxidase inhibitor to the vascular adventitia attenuates medial smooth muscle hypertrophy." in: Circulation research, Vol. 95, Issue 6, pp. 587-94, 2004 (PubMed).

Nagotani, Hayashi, Sato et al.: "Reduction of cerebral infarction in stroke-prone spontaneously hypertensive rats by statins associated with amelioration of oxidative stress." in: Stroke; a journal of cerebral circulation, Vol. 36, Issue 3, pp. 670-2, 2005 (PubMed).

Shiraishi, Naito: "Increased expression of Leydig cell haem oxygenase-1 preserves spermatogenesis in varicocele." in: Human reproduction (Oxford, England), Vol. 20, Issue 9, pp. 2608-13, 2005 (PubMed).

Tanito, Elliott, Kotake et al.: "Protein modifications by 4-hydroxynonenal and 4-hydroxyhexenal in light-exposed rat retina." in: Investigative ophthalmology & visual science, Vol. 46, Issue 10, pp. 3859-68, 2005 (PubMed).

Hirono, Yoshimoto, Suzuki et al.: "Angiotensin II receptor type 1-mediated vascular oxidative stress and proinflammatory gene expression in aldosterone-induced hypertension: the possible role of local renin-angiotensin system." in: Endocrinology, Vol. 148, Issue 4, pp. 1688-96, 2007 (PubMed).

Tanito, Kaidzu, Anderson: "Delayed loss of cone and remaining rod photoreceptor cells due to impairment of choroidal circulation after acute light exposure in rats." in: Investigative ophthalmology & visual science, Vol. 48, Issue 4, pp. 1864-72, 2007 (PubMed).

Kodai, Takemura, Minamiyama et al.: "S-allyl cysteine prevents CCl(4)-induced acute liver injury in rats." in: Free radical research, Vol. 41, Issue 4, pp. 489-97, 2007 (PubMed).

Choi, Ryu, Kim et al.: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, 2007 (PubMed).

Kurimoto, Kawano, Tsuji et al.: "Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome." in: The Journal of rheumatology, Vol. 34, Issue 10, pp. 2035-43, 2007 (PubMed).

Amidi, French, Chung et al.: "M-30 and 4HNE are sequestered in different aggresomes in the same hepatocytes." in: Experimental and molecular pathology, Vol. 83, Issue 3, pp. 296-300, 2007 (PubMed).

Kurashige, Abe, Furusu et al.: "Renoprotective effect of azelnidipine in rats." in: Biological & pharmaceutical bulletin, Vol. 31, Issue 12, pp. 2237-44, 2008 (PubMed).

Tskitishvili, Sharentuya, Temma-Asano et al.: "Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells." in: Molecular human reproduction, Vol. 16, Issue 3, pp. 188-99, 2010 (PubMed).

Ogawa, Fujii, Yoshizato et al.: "A human-type nonalcoholic steatohepatitis model with advanced fibrosis in rabbits." in: The American journal of pathology, Vol. 177, Issue 1, pp. 153-65, 2010 (PubMed).

Raz, Zhang, Zhou et al.: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, 2010 (PubMed).

Wakamatsu, Dogru, Ayako et al.: "Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease." in: Molecular vision, Vol. 16, pp. 2465-75, 2010 (PubMed).