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Alternatives Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|27 references available|
|Quantity||20 ug (Variants)|
|Price||421.91 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Immunogen||4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.|
|Description||During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. This antibody shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.|
|Specificity||Specific for 4HNE-Lysine adduct in all species. This antibody has almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.|
|Application Notes||IHC, Western and/or ELISA. Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.|
|Purification||Ammonium sulphate purified IgG|
|Buffer||Lyophilized as 100 ug/mL IgG in 50mM Tris buffered saline (TBS)|
|Storage||Less than -20°C, stable for 5 years Recinstitute with 200µl of distilled water.|
|Restrictions||For Research Use only|
|High-power view of colorectal carcinoma cells. Adenocarcinoma (arrows) and nontumorous epithelial cells (star x240). (S. Kondo et al. Free Radical Biology & Medicine 27 pp401-410, 1999)|
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|Applications||ELISA (1), Immunohistochemistry (IHC) (1), Western Blotting (WB) (1)|