PCNA antibody
| Antigen |
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| Clonality |
Monoclonal (PC10) |
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Host
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Reactivity
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 Alternatives Human, Mouse (Murine), Rat (Rattus), Insect, Yeast (Saccharomyces Cerevisiae)
Human (143), Rat (Rattus) (92), Mouse (Murine) (67), Cow (Bovine) (39), Dog (Canine) (39), Rabbit (36), Chicken (19), Horse (Equine) (6), Pig (Porcine) (5), Vertebrates (5), Fish (4), Yeast (Pichia pastoris) (4), Amphibian (3), Fruit Fly (Drosophila melanogaster) (3), Insect (3), Mammalian (3), Yeast (Saccharomyces Cerevisiae) (2), Monkey (1), Primate (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Application
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Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (104), Flow Cytometry (FACS) (69), Immunofluorescence (IF) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (60), Immunoprecipitation (IP) (31), ELISA (20), Immunohistochemistry (IHC) (16), Enzyme Immunoassay (EIA) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunocytochemistry (ICC) (5), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
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| Catalog no. |
ABIN336898 |
| Quantity |
7 ml (Variants) |
| Price |
100.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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Recombinant rat PCNA protein
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Format
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Liquid (prediluted)
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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PC10
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Description
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Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase delta in eukaryotic cells. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle It is increased during late G1 phase and S phase of the cell cycle and declines during G2 and M phases.
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Specificity
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Molecular Weight: ~30 kDa.
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Application Notes
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IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1 For IHC dilute conc. antibodies 1:50-1:200, using streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. FFPE tissue section requires antigen retriever (boiling tissue in 10 mM citrate, pH 6.0 for 20 mins, followed by cooling at RT for 20 mins). WB: 1: 100-1:400. The optimum dilution should be determined by the individual lab. Positive Control: Human tonsil, lymph node. Cellular Localization: Nuclear.
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Buffer
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The antibodies are supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.
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Storage
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2-8°C
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Research Area
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Proliferation Markers
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Restrictions
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For Research Use only
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Alternatives for antigen "PCNA", type "Antibodies"
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Hosts
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Mouse (101), Rabbit (82)
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Reactivities
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Human (143), Rat (Rattus) (92), Mouse (Murine) (67), Cow (Bovine) (39), Dog (Canine) (39), Rabbit (36), Chicken (19), Horse (Equine) (6), Pig (Porcine) (5), Vertebrates (5), Fish (4), Yeast (Pichia pastoris) (4), Amphibian (3), Fruit Fly (Drosophila melanogaster) (3), Insect (3), Mammalian (3), Yeast (Saccharomyces Cerevisiae) (2), Monkey (1), Primate (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (104), Flow Cytometry (FACS) (69), Immunofluorescence (IF) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (60), Immunoprecipitation (IP) (31), ELISA (20), Immunohistochemistry (IHC) (16), Enzyme Immunoassay (EIA) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunocytochemistry (ICC) (5), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
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Conjugates
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FITC (14), Biotin (10), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), PE (4), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), APC (1), RPE (1)
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