PIK3CA antibody (AA 878-891)

Catalog No. ABIN337177

This Goat Polyclonal antibody specifically detects PIK3CA in IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit, Dog, Bat, Hamster, Horse and Monkey.

Quick Overview for PIK3CA antibody (AA 878-891) (ABIN337177)

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Bat, Hamster, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This PIK3CA antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIK3CA Antibody

Binding Specificity: AA 878-891

Specificity: Human PIK3CA.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%) Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HQWLKDKNKGEIYD from an internal region of human PIK3CA (NP_006209.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%), Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PIK3CA

Target: PIK3CA (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA))

Alternative Name: PIK3CA / PI3K

Background: Name/Gene ID: PIK3CA
Family: Non-Protein Kinase

Synonyms: PIK3CA, MCAP, p110-alpha, p110alpha, PI3K, PI3-kinase p110 subunit alpha, PI3-kinase subunit alpha, PI3Kalpha, MCM, MCMTC, PI3K-alpha, PtdIns-3-kinase subunit alpha, CLOVE

Gene ID: 5290

NCBI Accession: NP_006209

UniProt: P42336

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling