NFKB1 antibody (AA 1-15)

Catalog No. ABIN337304

The Rat Monoclonal anti-NFKB1 antibody is suitable to detect NFKB1 in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for NFKB1 antibody (AA 1-15) (ABIN337304)

Target: NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This NFKB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFKB1 Antibody

Binding Specificity: AA 1-15

Specificity: Recognizes p50 subunit of human NF-kB (epitope in the region aa 1-15).

Purification: Protein G purified

Immunogen: Recombinant human NF- B (p50 subunit).

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for NFKB1

Target: NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))

Alternative Name: NFKB1

Background: Name/Gene ID: NFKB1
Family: Apoptosis

Synonyms: NFKB1, Ebp- 1, EBP-1, NF-KappaB p50, NFkappaB, NFKB-p50, KBF1, NF-kappa-B, NF-kappaB, NF-kB1, NFKB-p105, p105, p50, DNA binding factor KBF1, DNA-binding factor KBF1, NF-kappabeta

Gene ID: 4790

UniProt: P19838

Pathways: p53 Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins