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NFKB1 antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1) AA 860-969 Primary Antibody

NFKB1 Reactivity: Human IF, IHC (p), PLA, ELISA, WB Host: Mouse Monoclonal 4A11 unconjugated
Independent Validation (1 validation)
Catalog No. ABIN561981
$611.43
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100 μg
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  • Target See all NFKB1 Antibodies
    NFKB1
    Binding Specificity
    • 39
    • 30
    • 29
    • 29
    • 18
    • 16
    • 16
    • 16
    • 15
    • 14
    • 8
    • 7
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 860-969
    Reactivity
    • 364
    • 195
    • 166
    • 15
    • 12
    • 10
    • 9
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 357
    • 25
    • 2
    • 1
    Mouse
    Clonality
    • 352
    • 33
    Monoclonal
    Conjugate
    • 179
    • 22
    • 17
    • 16
    • 16
    • 16
    • 16
    • 16
    • 16
    • 12
    • 10
    • 9
    • 9
    • 9
    • 9
    • 9
    • 2
    • 2
    This NFKB1 antibody is un-conjugated
    Application
    • 201
    • 120
    • 118
    • 118
    • 115
    • 109
    • 75
    • 61
    • 33
    • 28
    • 14
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA), ELISA, Western Blotting (WB)
    Purpose
    Mouse monoclonal antibody raised against a partial recombinant NFKB1.
    Cross-Reactivity
    Human
    Immunogen

    immunogen: NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Immunogen Sequence: MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI

    Clone
    4A11
    Isotype
    IgG1 kappa
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Product Quality tested by: Antibody Reactive Against Recombinant Protein.

    Restrictions
    For Research Use only
  • Validation #104071 (Immunofluorescence)
    'Independent Validation' Badge
    by
    Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum
    No.
    #104071
    Date
    11/21/2019
    Antigen
    NFKB1
    Lot Number
    11067-4A11
    Method validated
    Immunofluorescence
    Positive Control

    U-937 human monocytic cell line

    Negative Control

    No primary antibody control

    Notes

    Passed. ABIN561981 specifically stains NF-κB1 in U-937 human monocytic cells.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN561981
    Secondary Antibody
    AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214)
    Full Protocol
    • Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO2 in 300µL in 24-well plates (Sarstedt AG&Co. KG).
    • Use cytospin to spin cells onto slide.
    • Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
    • Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
    • Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
    • Wash cells 3x for 5min with PBS.
    • Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
    • Incubate cells with primary mouse anti-human NF-κB1/p50 antibody (antibodies-online, ABIN561981, lot 11067-4A11) diluted 1:50 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Incubate cells with secondary AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214) diluted 1:400 in 1x Duolink Antibody diluent for 1h at RT in the dark.
    • Wash cells 2x for 5min with PBS.
    • Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
    • Image acquisition with an Olympus widefield microscope.
    Experimental Notes

    Strong green staining dependent on the presence of primary antibody indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence.

  • Buffer
    In 1x PBS, pH 7.4
    Handling Advice
    Aliquot to avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Target
    NFKB1
    Alternative Name
    NFKB1 (NFKB1 Products)
    Synonyms
    EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkappaB, p105, p50, NF-kB, NF-KB1, ebp-1, kbf1, nf-kappa-b, nf-kappaB, nfkb-p105, nfkb-p50, NF-kappaB1, p50/p105, BICDR-1, bicdr1, ccdc64, nuclear factor kappa B subunit 1, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 L homeolog, nuclear factor NF-kappa-B p105 subunit, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, NFKB1, Nfkb1, nfkb1.L, CpipJ_CPIJ012236, nfkb1
    Background
    Full Gene Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
    Synonyms: DKFZp686C01211,EBP-1,KBF1,MGC54151,NF-kappa-B,NFKB-p105,NFKB-p50,p105,p50
    Gene ID
    4790
    Pathways
    p53 Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins
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