SMUG1 antibody (AA 2-14)

Catalog No. ABIN337309

Product Details anti-SMUG1 Antibody

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Binding Specificity: AA 2-14

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This SMUG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SMUG1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Monkey (100%) Gorilla, Gibbon (92%) Horse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide PQAFLLGSIHEPA-C from the N-terminus of human SMUG1 (NP_055126.1). Percent identity by BLAST analysis: Human, Monkey (100%), Gorilla, Gibbon (92%), Horse (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SMUG1

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Alternative Name: SMUG1 (SMUG1 Products)

Synonyms: SMUG1 antibody, xsmug1 antibody, 1200013B09Rik antibody, A930006H09Rik antibody, C85220 antibody, FDG antibody, HMUDG antibody, UNG3 antibody, single-strand-selective monofunctional uracil-DNA glycosylase 1 antibody, single-strand selective monofunctional uracil DNA glycosylase antibody, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog antibody, SMUG1 antibody, smug1 antibody, smuG1 antibody, smuG antibody, LOC5577584 antibody, CpipJ_CPIJ002767 antibody, Smug1 antibody, smug1.L antibody

Background: Name/Gene ID: SMUG1

Synonyms: SMUG1, HMUDG, FDG, UNG3

Gene ID: 23583

NCBI Accession: NP_055126

UniProt: Q53HV7

Pathways: DNA Damage Repair