Collagen Type I antibody

Catalog No. ABIN344703

The Rabbit Polyclonal anti-Collagen Type I antibody (ABIN344703) specifically detects Collagen Type I in WB, IHC, ELISA, IHC (fro) and IP. The antibody is reactive with Human samples.

Quick Overview for Collagen Type I antibody (ABIN344703)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Product Details anti-Collagen Type I Antibody

Specificity: Recognizes most mammalian Type I collagens. Demonstrates negligible cross-reactivity with Type II, III, IV, V or VI collagens (<1 % ). Demonstrates negligible non-specific cross-reactivity with anti-collagen antibodies directed against other human serum proteins and non-collagen extracellular matrix proteins.

Purification: Immunoaffinity purified

Immunogen: Collagen Type I from human and bovine placenta.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:2500 - 1:25000), IHC, IHC-Fr, IP, WB (1:2500 - 1:25000)

Usage: Suitable for use in ELISA, Immunoprecipitation, native PAGE, Immunohistochemistry and Western Blot. Western Blot: 1:2500-1:25000. ELISA: 1:2500-1:25000 (using 1 μg of Collagen I). Immunohistochemistry (frozen tissue): 1:25-1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 0.01 % sodium azide, 40 % glycerol. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen I