Collagen Type I antibody (Biotin)

Catalog No. ABIN135047

Product Details anti-Collagen Type I Antibody (Biotin)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is conjugated to Biotin

Application: ELISA

Specificity: Reacts with conformational determinants on type I collagen Referenced species reactivities include - Human 1-5,10,20,26,27,29,31-34 Chicken 17 Hamster 11,18 Mouse 24,27,28 Chinchilla 19 Porcine 5,21 Rat 5,13-15,23,25,27,35 Elephant 8 Rabbit 16,30 Bovine 6,20 Feline 9 Sheep 7 Canine 5,12 Guinea Pig 16 Tree Shrew 22

Cross-Reactivity (Details): Exhibits < 10 % cross reactivity with collagen type II, III, IV, V and VI. The antibody has not been tested for reactivity with other ECM proteins (e.g., laminin, fibronectin).

Characteristics: Goat Anti-Type I Collagen-BIOT

Purification: Purification Method: Affinity chromatography on type I collagen covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA
• Other referenced applications include - IHC-PS , IHC-FS , ICC , EM , FC , WB , IP
• Working Dilutions: ELISA BIOT conjugate 1:1,000 - 1:4,000 FLISA FITC and AF488 conjugates 1:200 - 1:400

Sample Volume: 0,5 mL

Restrictions: For Research Use only

Handling

Concentration: 0.4 mg/mL

Buffer: 0.2 mg in 0.5 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Collagen Type I antibody (Biotin) (ABIN135047)

Chen, Chen, Liang, Gu, Zhou, Xie, Lv, Wang, Li, Mao, Sun, Zuo, Miao, Jin: "TGF-β1/IL-11/MEK/ERK signaling mediates senescence-associated pulmonary fibrosis in a stress-induced premature senescence model of Bmi-1 deficiency." in: Experimental & molecular medicine, Vol. 52, Issue 1, pp. 130-151, (2020) (PubMed).

Jeffers, Qin, Owens, Koenig, Komatsu, Giles, Schmitt, Idell, Tucker: "Glycogen Synthase Kinase-3β Inhibition with 9-ING-41 Attenuates the Progression of Pulmonary Fibrosis." in: Scientific reports, Vol. 9, Issue 1, pp. 18925, (2019) (PubMed).

Lee, Lee, Chae, Jang, Lee, Son: "Fully Dedifferentiated Chondrocytes Expanded in Specific Mesenchymal Stem Cell Growth Medium with FGF2 Obtains Mesenchymal Stem Cell Phenotype In Vitro but Retains Chondrocyte Phenotype In Vivo." in: Cell transplantation, Vol. 26, Issue 10, pp. 1673-1687, (2018) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Type I Collagen (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody