Collagen Type I antibody (Biotin)

Catalog No. ABIN344705

The Rabbit Polyclonal anti-Collagen Type I antibody is suitable to detect Collagen Type I in samples from Human. It has been validated for WB, IHC, ELISA and IP.

Quick Overview for Collagen Type I antibody (Biotin) (ABIN344705)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-Collagen Type I Antibody (Biotin)

Specificity: Recognizes Collagen Type I. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- fixed, paraffin embedded tissues. Recognizes most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens detected by ELISA against purified standards. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Purification: Immunoaffinity purified

Immunogen: Collagen Type I from human and bovine placenta.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:3000 - 1:6000), IHC (1:50 - 1:200), IP, WB (1:3000 - 1:6000)

Usage: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation. Western Blot: 1:3000-1:6000. ELISA: 1:3000-1:6000. Immunohistochemistry: 1:50-1:200. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with100 μL sterile 40-50 % glycerol, ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA,  pH 8.0, 10 mg/mL BSA, 0.01 % sodium azide. Reconstitute with100 μLsterile 40-50 % glycerol, ddH2O.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen I