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Tumor Protein P53 (TP53) (pSer20) antibody

Antigen

Tumor Protein P53 (TP53)
Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Epitope
Alternatives

pSer20
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine)
Application
Alternatives ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Catalog no. ABIN349228
Quantity 100ug  (100µg/100µl)  (Variants)
Price 406.67 $   Plus shipping costs $35.00
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anti-Tumor Protein P53 (TP53) (pSer20) antibody

anti-Tumor Protein P53 (TP53) (pSer20) antibody (2)

Additional Information
Application Details Images Alternatives Secondary Antibodies Variants back to top

Alternative name P53 (Phospho-Ser20)
Swiss-Prot P04637
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human P53 around the phosphorylation site of serine 20 (T-F-S P -D-L).
Isotype IgG  (Matching secondary antibodies)
Description Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Specificity P53 (phospho-Ser20) antibody detects endogenous levels of P53 only when phosphorylated at serine 20.

Application Details
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Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
Concentration 100µg/100µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20° C /1 year
Restrictions For Research Use only

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (302), Mouse (102), Sheep (3)
Reactivities Human (390), Mouse (Murine) (67), Rat (Rattus) (62), Monkey (5), Cat (Feline) (3), Cow (Bovine) (3), Dog (Canine) (3), Hamster (3), Horse (Equine) (3), Primate (2), Primates (2), Sheep (Ovine) (2), Bird (Avian) (1), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (337), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (112), Immunohistochemistry (IHC) (80), Immunofluorescence (IF) (76), Enzyme Immunoassay (EIA) (68), ELISA (67), Immunoprecipitation (IP) (53), Flow Cytometry (FACS) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Immunocytochemistry (ICC) (11), Dot Blot (Dot) (10), ELISA (Detection) (5), Transfected cell culture (TCC) (3), Chromatin Immunoprecipitation (ChiP) (2), Radioimmunoassay (RIA) (2)
Conjugates FITC (7), Biotin (2), HRP (1), PE (1)
Epitopes pSer315 (12), pSer37 (12), pSer46 (11), pSer33 (10), pThr18 (10), pSer6 (9), pSer392 (8), pSer15 (7), pSer20 (6), AA 371-380 (5), pSer32/Ser36 (5), AA 20-25 (4), pSer1232 (4), AA 18-30 (3), C-Term (3), pSer9 (3), S315 (2), S37 (2), S46 (2), T55 (2), pSer376 (2), pSer378 (2), pThr55 (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), AA 46-55 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), acLys120 (1), acLys382 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), phospho (1)