Autophagy Related 12 (ATG12) antibody

Details for Product No. ABIN350094
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Antigen
Synonyms APG12, APG12L, FBR93, HAPG12, 4931423H11Rik, A330058M13Rik, Apg12l, Atg12l
Reactivity
Rat (Rattus), Mouse (Murine)
(109), (46), (43), (12), (12), (12), (5), (1), (1)
Host
Sheep
(105), (15), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(101), (62), (58), (16), (15), (14), (10), (5), (4), (4), (3), (1), (1)
Pubmed 5 references available
Quantity 500 μg
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Catalog No. ABIN350094
454.67 $
Plus shipping costs $45.00

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Immunogen A synthetic peptide from rat, mouse ATG12 (APG12, APG12L, HAPG12) conjugated to an immunogenic carrier protein has been used as the immunogen.
Isotype IgG
Specificity Specific for ATG12.
Alternative Name ATG12
Background Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved inautophagy. Following conjugation to ATG5, the conjugate participates in the formation of autophagosome. Also known as: APG12-like, HAPG12, APG12, ATG12autophagy related 12 homolog.
Application Notes Use at a concentration of 10-50 µg/ml.
The optimal concentration should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Tanida, Tanida-Miyake, Komatsu et al.: "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13739-44, 2002 (PubMed).

Tanida, Nishitani, Nemoto et al.: "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing." in: Biochemical and biophysical research communications, Vol. 296, Issue 5, pp. 1164-70, 2002 (PubMed).

Mizushima, Yoshimori, Ohsumi: "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method." in: FEBS letters, Vol. 532, Issue 3, pp. 450-4, 2002 (PubMed).

Mizushima, Kuma, Kobayashi et al.: "Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate." in: Journal of cell science, Vol. 116, Issue Pt 9, pp. 1679-88, 2003 (PubMed).

Prigione, Cortopassi: "Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12." in: Autophagy, Vol. 3, Issue 4, pp. 377-80, 2007 (PubMed).

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