GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (C-Term)

Details for Product anti-GAPDH Antibody No. ABIN350330, Supplier: Log in to see
Antigen
  • CG8893
  • Dmel\\CG8893
  • GA3PDH
  • GADPH
  • GAP
  • GAPDH
  • GAPDH II
  • GAPDH2
  • Gapd
  • Gapdh
  • Gapdh-2
  • Gapdh13F
  • GAPD
  • g3pd
  • gapd
  • GB14798
  • GAPC-2
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • T6J4.17
  • T6J4_17
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • BEST:GH12586
  • CG12055
  • Dmel\\CG12055
  • GAPDH I
  • GAPDH-1
  • GAPDH1
  • GAPDHI
  • Gapdh-1
  • Gapdh43E
  • gadph
  • gapdh
  • gapdh-1
  • gh12586
  • KNC-NDS6
  • G3PD
  • cb609
  • mg:bb02e05
  • wu:fb33a10
  • wu:ft80f05
  • G3PDH
  • Glyceraldehyde 3 phosphate dehydrogenase 2
  • glyceraldehyde-3-phosphate dehydrogenase
  • glyceraldehyde 3-phosphate dehydrogenase
  • similar to Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  • glyceraldehyde-3-phosphate dehydrogenase, type I
  • Glyceraldehyde 3 phosphate dehydrogenase 1
  • glyceraldehyde-3-phosphate dehydrogenase-like
  • olfactory receptor, family 8, subfamily K, member 3-like
  • Gapdh2
  • GAPDH
  • gapdh
  • Gapdh
  • GAPC2
  • gapDH
  • Gapdh1
  • LOC614985
Epitope
C-Term
109
34
32
22
17
10
9
9
8
8
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7
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6
6
6
5
5
5
5
5
5
5
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4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus), Mouse (Murine), Human
742
425
341
161
134
84
78
60
60
58
55
50
49
29
28
26
23
23
18
17
15
15
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13
10
10
9
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6
6
6
5
5
5
4
4
3
3
3
3
1
1
1
1
1
1
1
Host
Sheep
389
357
58
30
18
1
Clonality
Polyclonal
Conjugate
This GAPDH antibody is un-conjugated
55
42
22
15
13
13
12
11
11
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10
8
8
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7
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6
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3
2
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2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
771
346
298
216
213
163
55
34
30
23
17
16
13
11
7
7
6
6
4
2
2
1
1
1
1
1
1
Options
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Immunogen A synthetic peptide from c-terminal of rat GAPDH conjugated to blue carrier protein was used as the antigen. The antigen is homologous in mouse and shares 90% identity with human sequence.
Isotype IgG
Specificity Specific for GAPDH.
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background Function: May bind tRNA. Has uracil-DNA-glycosylase activity. May activate transcription in neurons. Functions as a killing protein in apoptosis when over-expressed.
Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. PATHWAY: Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit: Homotetramer.
Subcellular location: Cytoplasm.
Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Also known as: 38 kDa BFA dependent ADP ribosylation substrate, BARS-38, Glyceraldehyde-3-phosphate dehydrogenase.
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 250 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (C-Term) (ABIN350330) WB on human neutrophil lysate using Sheep antibody to c-terminal of GAPDH (Glyceralde...
Background publications Ballif, Carey, Sunyaev, Gygi: "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." in: Journal of proteome research, Vol. 7, Issue 1, pp. 311-8, 2008 (PubMed).

Kullberg, Nilsson, Arnason, Harley, Janke: "Housekeeping genes for phylogenetic analysis of eutherian relationships." in: Molecular biology and evolution, Vol. 23, Issue 8, pp. 1493-503, 2006 (PubMed).

Mohr, Stamler, Bruene: "Posttranslational modification of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosylation and subsequent NADH attachment." in: The Journal of biological chemistry, Vol. 271, Issue 8, pp. 4209-14, 1996 (PubMed).

Fort, Marty, Piechaczyk, el Sabrouty, Dani, Jeanteur, Blanchard: "Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family." in: Nucleic acids research, Vol. 13, Issue 5, pp. 1431-42, 1985 (PubMed).

Tso, Sun, Kao, Reece, Wu: "Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene." in: Nucleic acids research, Vol. 13, Issue 7, pp. 2485-502, 1985 (PubMed).