Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7) (4th Cytoplasmic Loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7)

Synonyms: CHAK, CHAK1, LTRPC7, FLJ20117, FLJ25718, TRP-PLIK, Ltpr7, Ltrpc7, LTrpC-7, TRPPLIK, 2310022G15Rik, 4833414K03Rik, 5033407O22Rik, cb495, TRPM7, Chak, Trp-plik, chak, chak1, ltrpc7, trp-plik

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351218

Additional Information

Alternative name: TRPM7 (LTRPC7)

UniProt: Q96QT4

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human TRPM7 (LTRPC7) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Isotype: IgG

Description: TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants. FUNCTION: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1). CATALYTIC ACTIVITY: ATP + a protein: ADP + a phosphoprotein. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. Forms heterodimers with TRPM6. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Transient receptor potential cation channel subfamily M member 7, long transient receptor potential channel 7, LTrpC7, channel-kinase 1, CHAK1, LTRPC7, CHAK, FLJ20117, FLJ25718, TRP-PLIK.

Specificity: Appears to be specific for TRPM7.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IF on PC12 cells at a concentration of 30 µg/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG (ABIN351218), DAPI counterstained.

IF on PC12 cells at a concentration of 30 µg/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG (ABIN351218), DAPI counterstained appearing in blue.

Publications

Schmitz, Perraud, Johnson et al.: "Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7." in: Cell, Vol. 114, Issue 2, pp. 191-200, 2003 (PubMed).

Ryazanova, Dorovkov, Ansari et al.: "Characterization of the protein kinase activity of TRPM7/ChaK1, a protein kinase fused to the transient receptor potential ion channel." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3708-16, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Chubanov, Waldegger, Mederos y Schnitzler et al.: "Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 9, pp. 2894-9, 2004 (PubMed).

Dorovkov, Ryazanov: "Phosphorylation of annexin I by TRPM7 channel-kinase." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 50643-6, 2004 (PubMed).

Hermosura, Nayakanti, Dorovkov et al.: "A TRPM7 variant shows altered sensitivity to magnesium that may contribute to the pathogenesis of two Guamanian neurodegenerative disorders." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 32, pp. 11510-5, 2005 (PubMed).