Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN356280

This anti-Smooth Muscle Actin antibody is a Mouse Monoclonal antibody detecting Smooth Muscle Actin in IF, IHC (p) and IHC (fro). Suitable for Human, Rat, Mouse, Chicken, Rabbit, Cow and Monkey.

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN356280)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Chicken, Rabbit, Cow, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Aasm 204 (1A4)

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Specificity: This antibody recognises ACTIN alpha Smooth Muscle.
Cellular Localization: Cytoplasmic.

Characteristics: Synonyms: Alpha-actin-2, ACTVS, GIG46, Cell growth-inhibiting gene 46 protein

Purification: Purified

Immunogen: N-terminal decapeptide of alpha smooth muscle isoform of actin, acetylated at theN-terminus. Remarks: Epitope: Acetyl group with N- terminal four amino acids of peptide chain of alphasmooth muscle.

Isotype: IgG2a

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Recommended Positive Control: Human uterus, gastrointestinal tissue, Leiomyosarcoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Storethe antibody undiluted at 2-8 °Cfor one month or (in aliquots) at -28 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Background: Actin is a globular, roughly 42- kDa protein found in all eukaryotic cells. Actin participates in many important cellular functions, including muscle contraction cell motility cell division and cytokinesis vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Expression of smooth muscle alpha actin is regulated by hormones and cell proliferation and is altered by pathological conditions including onogenic transformation and atherosclerosis.Synonyms: ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development