DICER1 antibody (Middle Region)

Catalog No. ABIN357311

The Rabbit Polyclonal anti-DICER1 antibody is suitable to detect DICER1 in samples from C. elegans. It has been validated for WB and EIA.

Quick Overview for DICER1 antibody (Middle Region) (ABIN357311)

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Reactivity: C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DICER1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-DICER1 Antibody

Binding Specificity: AA 1010-1040, Middle Region

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 1010~1040 amino acids from the central region of C. elegans Dcr-1 . Genename: dcr-1

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for DICER1

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Background: The Dcr-1 gene encodes a bidentate ribonuclease that is homologous to E. coli RNAse III. Dcr-1 is required both for RNA interference and for synthesis of small developmental RNAs. Dcr-1 interacts in vivo with RDE-4, a double-stranded RNA (dsRNA) binding protein required for RNAi that interacts with trigger dsRNAs and may function to deliver dsRNAs to Dcr-1 for endonucleolytic processing.Synonyms: Caenorhabditis elegans

Molecular Weight: 210924 Da

Gene ID: 176138, 6239

UniProt: P34529

Pathways: Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization