DICER1 antibody (AA 1-195)

Catalog No. ABIN3043347

Cited in 2+ publications. The Rabbit Polyclonal anti-DICER1 antibody (ABIN3043347) specifically detects DICER1 in WB, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DICER1 antibody (AA 1-195) (ABIN3043347)

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DICER1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-DICER1 Antibody

Binding Specificity: AA 1-195

Purpose: Anti-Dicer/DICER1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Dicer/DICER1 Antibody (ABIN3043347). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-DICER1 antibody (ABIN3043347)

Gao, Wang, Chen, Feng, Yang, Feng, Yang, Ye, Zhang: "Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells." in: Acta pharmacologica Sinica, Vol. 37, Issue 7, pp. 898-907, (2017) (PubMed).

Gao, Wang, Feng, Feng, Zheng, Yang, Xiao, Lu, Ye, Zhang: "A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level." in: Biochemical and biophysical research communications, Vol. 459, Issue 2, pp. 306-312, (2015) (PubMed).

Target Details for DICER1

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Alternative Name: DICER1

Background:

Synonyms: Endoribonuclease Dicer,3.1.26.3,Helicase with RNase motif,Helicase MOI,DICER1,DICER, HERNA, KIAA0928,

Background: Dicer (DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

Sequence Similarities: Belongs to the helicase family. Dicer subfamily.

Molecular Weight: 250 kDa

Gene ID: 23405

Pathways: Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization