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DICER1 antibody (AA 1-195)

DICER1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3043347
  • Target See all DICER1 Antibodies
    DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
    Binding Specificity
    • 15
    • 12
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-195
    Reactivity
    • 48
    • 23
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 38
    • 24
    • 1
    Rabbit
    Clonality
    • 38
    • 25
    Polyclonal
    Conjugate
    • 35
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DICER1 antibody is un-conjugated
    Application
    • 51
    • 18
    • 16
    • 14
    • 13
    • 13
    • 13
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Endoribonuclease Dicer(DICER1) detection. Tested with WB, IHC-P in Human.
    Gene Name: dicer 1, ribonuclease type III
    Protein Name: Endoribonuclease Dicer
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human Dicer recombinant protein (Position: M1-N195). Human Dicer shares 94% amino acid (aa) sequence identity with mouse Dicer.
    Isotype
    IgG
    Top Product
    Discover our top product DICER1 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Dicer is approximately 0.25 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Gao, Wang, Chen, Feng, Yang, Feng, Yang, Ye, Zhang: "Post-transcriptional modulation of protein phosphatase PPP2CA and tumor suppressor PTEN by endogenous siRNA cleaved from hairpin within PTEN mRNA 3'UTR in human liver cells." in: Acta pharmacologica Sinica, Vol. 37, Issue 7, pp. 898-907, (2017) (PubMed).

    Gao, Wang, Feng, Feng, Zheng, Yang, Xiao, Lu, Ye, Zhang: "A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level." in: Biochemical and biophysical research communications, Vol. 459, Issue 2, pp. 306-312, (2015) (PubMed).

  • Target
    DICER1 (Dicer 1, Ribonuclease Type III (DICER1))
    Alternative Name
    DICER1 (DICER1 Products)
    Synonyms
    dcr1 antibody, dicer antibody, herna antibody, DICER1 antibody, dicer1 antibody, 1110006F08Rik antibody, D12Ertd7e antibody, mKIAA0928 antibody, DCR1 antibody, Dicer antibody, HERNA antibody, MNG1 antibody, DICER antibody, fc39d11 antibody, fc74b05 antibody, wu:fc39d11 antibody, wu:fc74b05 antibody, dicer 1, ribonuclease III antibody, endoribonuclease Dicer antibody, dicer 1, ribonuclease III S homeolog antibody, dicer 1, ribonuclease type III antibody, dicer 1 ribonuclease III antibody, DICER1 antibody, CpipJ_CPIJ010534 antibody, dicer1 antibody, dicer1.S antibody, Dicer1 antibody
    Background
    Dicer(DICER1), also known as endoribonuclease Dicer or helicase with RNase motif, is an enzyme that in humans is encoded by the DICER1 gene. It is mapped to 14q32.13. The DICER1 gene, a member of the ribonuclease III (RNaseIII) family, is involved in the generation of microRNAs (miRNAs), which modulate gene expression at the posttranscriptional level. DICER1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus DICER, also known as helicase-MOI, is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. In addition, DICER1 is required for formation of the RNA induced silencing complex (RISC). It also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.

    Synonyms: DCR antibody|DCR1 antibody|Dcr1 homolog (Drosophila) antibody|Dicer 1 antibody|Dicer 1 ribonuclease type III antibody|Dicer antibody|DICER_HUMAN antibody|Dicer1 antibody|Double-strand-specific ribonuclease antibody|Endoribonuclease Dicer antibody|Helicase moi antibody|Helicase with RNAse motif antibody|HERNA antibody|KIAA0928 antibody|MNG1 antibody|RMSE2 antibody
    Gene ID
    23405
    Pathways
    Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
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