DICER1 antibody (AA 1638-1899) (Biotin)

Catalog No. ABIN2483012

The Biotin-conjugated Mouse Monoclonal anti-DICER1 antibody (Clone S167-7) (ABIN2483012) specifically detects DICER1 in WB, ICC and IF. The antibody is reactive with Mouse samples.

Quick Overview for DICER1 antibody (AA 1638-1899) (Biotin) (ABIN2483012)

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This DICER1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S167-7

Product Details anti-DICER1 Antibody (Biotin)

Binding Specificity: AA 1638-1899

Specificity: Detects ~215 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN2483012 was sufficient for detection of Dicer in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for DICER1

Target: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Alternative Name: Dicer

Background: Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3' UTR of the target mRNA (3)

Gene ID: 192119

NCBI Accession: NP_683750

UniProt: Q8R418

Pathways: Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization