MIB1 antibody (N-Term)

Catalog No. ABIN357602

Product Details anti-MIB1 Antibody

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific to MIB1 (N-term). Predicted to cross react with Mouse and Zebrafish (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MIB.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Immunofluorescence: 1/20-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MIB1

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Alternative Name: MIB1 (MIB1 Products)

Synonyms: DIP-1 antibody, DIP1 antibody, LVNC7 antibody, MIB antibody, ZZANK2 antibody, ZZZ6 antibody, E430019M12Rik antibody, Mib antibody, mKIAA1323 antibody, dip-1 antibody, mib antibody, mindbomb antibody, cg5841 antibody, chunp6889 antibody, fe47f05 antibody, im:7148100 antibody, mib1 antibody, wu:fe47f05 antibody, CG5841 antibody, D-mib antibody, D-mib1 antibody, Dmel\CG5841 antibody, Dmib antibody, MIB1 antibody, Mib1 antibody, dmib1 antibody, l(3)72CDa antibody, l(3)72Db antibody, mib1/2 antibody, mind-bomb antibody, mindbomb E3 ubiquitin protein ligase 1 antibody, mindbomb E3 ubiquitin protein ligase 1 L homeolog antibody, mind bomb 1 antibody, MIB1 antibody, Mib1 antibody, mib1.L antibody, mib1 antibody

Background: MIB is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. This protein positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.Synonyms: DAPK-interacting protein 1, DIP1, E3 ubiquitin-protein ligase MIB1, KIAA1323, Mind bomb homolog 1, ZZANK2, Zinc finger ZZ type with ankyrin repeat domain protein 2

Molecular Weight: 110135 Da

Gene ID: 57534, 9606

UniProt: Q86YT6

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection