Fibrillarin antibody (Middle Region)
-
- Target See all Fibrillarin (FBL) Antibodies
- Fibrillarin (FBL)
-
Binding Specificity
- Middle Region
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Fibrillarin antibody is un-conjugated
-
Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects FBL (Center).
- Purification
- Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human FBL.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product FBL Primary Antibody
-
-
- Application Notes
-
ELISA: 1/1,000. Western Blot: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
- Target
- Fibrillarin (FBL)
- Alternative Name
- FBL (FBL Products)
- Synonyms
- FIB antibody, FLRN antibody, RNU3IP1 antibody, wu:fb37g12 antibody, zgc:56145 antibody, zgc:77130 antibody, FBL antibody, AL022665 antibody, CG9888 antibody, Dmel\\CG9888 antibody, Fibri antibody, GCR-6 antibody, GCR6 antibody, Pen59C5 antibody, fib antibody, pen59C5 antibody, MGC76139 antibody, fbl antibody, fibrillarin antibody, Fibrillarin antibody, putative fibrillarin antibody, fibrillarin S homeolog antibody, FBL antibody, fbl antibody, Fbl antibody, Fib antibody, fib1 antibody, LMJF_19_0100 antibody, fbl.S antibody
- Background
- FBL is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. This protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.Synonyms: FIB1, FLRN, Fibrillarin, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin
- Molecular Weight
- 33784 Da predicted from amino acid sequence. Theoretical pI: 10.18
- Gene ID
- 2091, 9606
- UniProt
- P22087
- Pathways
- Ribonucleoside Biosynthetic Process
-