FADD antibody (Fas (TNFRSF6)-Associated Via Death Domain) (pSer191)

Details for Product anti-FADD Antibody No. ABIN358126, Supplier: Log in to see
Antigen
  • MORT1
  • Mort1/FADD
  • Mort1
  • TNFRSF6
  • Fas (TNFRSF6)-associated via death domain
  • FADD
  • Fadd
Alternatives
anti-Mouse (Murine) FADD antibody for ELISA
Epitope
pSer191
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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98
Clonality
Polyclonal
Conjugate
This FADD antibody is un-conjugated
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Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S191 of mouse FADD.
Isotype Ig
Specificity This antibody detects FADD pSer191.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name FADD (FADD Antibody Abstract)
Background FADD is an apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.Synonyms: FAS-associated death domain protein, FAS-associating death domain-containing protein, GIG3, Growth-inhibiting gene 3 protein, MORT1, Mediator of receptor induced toxicity
Molecular Weight 22960 Da
Gene ID 14082, 10090
UniProt Q61160
Research Area Cancer, Apoptosis/Necrosis
Pathways Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-FADD antibody (Fas (TNFRSF6)-Associated Via Death Domain) (pSer191) (ABIN358126) anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (pSer191) antibody
 image for anti-FADD antibody (Fas (TNFRSF6)-Associated Via Death Domain) (pSer191) (ABIN358126) anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) (pSer191) antibody (Image 2)
Background publications Jeong, Bang, Lee, Park, Sim, Kim: "The solution structure of FADD death domain. Structural basis of death domain interactions of Fas and FADD." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 16337-42, 1999 (PubMed).

Hsu, Shu, Pan, Goeddel: "TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNF receptor 1 signal transduction pathways." in: Cell, Vol. 84, Issue 2, pp. 299-308, 1996 (PubMed).

Zhang, Winoto: "A mouse Fas-associated protein with homology to the human Mort1/FADD protein is essential for Fas-induced apoptosis." in: Molecular and cellular biology, Vol. 16, Issue 6, pp. 2756-63, 1996 (PubMed).