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RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pThr269)

Details for Product anti-RAF1 Antibody No. ABIN358313, Supplier: Log in to see
Antigen
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
Alternatives
anti-Human RAF1 antibody for Enzyme Immunoassay
Epitope
pThr269
75
60
56
39
35
34
27
26
26
26
24
23
20
20
19
17
17
16
16
15
15
14
13
13
13
12
9
8
8
6
6
6
6
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
781
334
315
18
18
10
5
4
4
2
2
1
1
Host
Rabbit
731
38
16
7
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
43
43
42
29
29
29
13
12
12
12
12
12
12
12
12
Application
Enzyme Immunoassay (EIA)
449
351
199
181
133
75
37
29
17
16
9
2
2
2
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T269 of human RAF1.
Isotype Ig
Specificity This antibody detects RAF1 pThr269.
Purification Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Molecular Weight 73052 Da
Gene ID 5894, 9606
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA: 1/1,000. Dot Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pThr269) (ABIN358313) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pThr269) antibody
Background publications Dasgupta, Sun, Wang et al.: "Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9527-41, 2004 (PubMed).

Ku, Fu, Omary: "Raf-1 activation disrupts its binding to keratins during cell stress." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 479-85, 2004 (PubMed).