ZMIZ1 antibody (C-Term)

Catalog No. ABIN358726

Product Details anti-ZMIZ1 Antibody

Target: ZMIZ1 (Zinc Finger, MIZ-Type Containing 1 (ZMIZ1))

Binding Specificity: AA 596-626, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZMIZ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to RAI17.

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide between 596~626 amino acids from the C-terminal region of Human RAI17

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ZMIZ1

Target: ZMIZ1 (Zinc Finger, MIZ-Type Containing 1 (ZMIZ1))

Alternative Name: ZMIZ1 / RAI17 (ZMIZ1 Products)

Synonyms: miz antibody, rai17 antibody, zimz1 antibody, zimp10 antibody, hzimp10 antibody, MIZ antibody, RAI17 antibody, TRAFIP10 antibody, ZIMP10 antibody, hZIMP10 antibody, BC065120 antibody, E330020C23 antibody, ENSMUSG00000072684 antibody, Gm10397 antibody, I920194n01 antibody, Rai17 antibody, Zimp10 antibody, wu:fb80e10 antibody, zgc:175159 antibody, zmiz1 antibody, zinc finger MIZ-type containing 1 antibody, zinc finger, MIZ-type containing 1 antibody, zinc finger, MIZ-type containing 1a antibody, ZMIZ1 antibody, zmiz1 antibody, Zmiz1 antibody, zmiz1a antibody

Background: Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes. RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain and ovaries. Within brain, highest expression is in amygdala. The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions. A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region. RAI17 expression is detected in various cancer cell lines. RAI17 colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.Synonyms: KIAA1224, PIAS-like protein Zimp10, Retinoic acid-induced protein 17, ZIMP10, Zinc finger MIZ domain-containing protein 1

Gene ID: 57178, 9606

UniProt: Q9ULJ6