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SPRY4 antibody (Sprouty Homolog 4) (C-Term)

Details for Product anti-SPRY4 Antibody No. ABIN359610, Supplier: Log in to see
Antigen
Epitope
C-Term
2
1
1
Reactivity
Human
4
2
2
2
2
1
Host
Rabbit
4
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
4
3
2
1
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPRY4.
Isotype Ig
Specificity This antibody reacts to SPRY4.
Purification Protein A column, followed by peptide affinity purification
Alternative Name Sprouty Homolog 4 / SPRY4
Background SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.Synonyms: Sprouty 4, Spry-4
Gene ID 81848, 9606
UniProt Q9C004
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-SPRY4 antibody (Sprouty Homolog 4) (C-Term) (ABIN359610) Western blot analysis of anti-SPRY4 Antibody (C-term) in Sk-Br-3 cell line lysates (3...
Background publications Rikova, Guo, Zeng, Possemato, Yu, Haack, Nardone, Lee, Reeves, Li, Hu, Tan, Stokes, Sullivan, Mitchell, Wetzel, Macneill, Ren, Yuan, Bakalarski, Villen, Kornhauser, Smith, Li, Zhou, Gygi, Gu et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007

Wolf-Yadlin, Hautaniemi, Lauffenburger, White: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007

Tsumura, Toshima, Leeksma, Ohashi, Mizuno: "Sprouty-4 negatively regulates cell spreading by inhibiting the kinase activity of testicular protein kinase." in: The Biochemical journal, Vol. 387, Issue Pt 3, pp. 627-37, 2005