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Sprouty Homolog 4 (SPRY4) (C-Term) antibody

Details for Product No. ABIN359610, Supplier: Login to see
Antigen
Epitope
C-Term
2
1
1
1
Reactivity
Human
5
2
2
2
2
1
Host
Rabbit
5
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
4
4
3
1
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SPRY4.
Isotype Ig
Specificity This antibody reacts to SPRY4.
Purification Protein A column, followed by peptide affinity purification
Alternative Name Sprouty Homolog 4 / SPRY4
Background SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.Synonyms: Sprouty 4, Spry-4
Gene ID 81848, 9606
UniProt Q9C004
Pathways
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Sprouty Homolog 4 (SPRY4) (C-Term) antibody (ABIN359610) Western blot analysis of anti-SPRY4 Antibody (C-term) in Sk-Br-3 cell line lysates (3...
Background publications Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Wolf-Yadlin, Hautaniemi, Lauffenburger et al.: "Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 14, pp. 5860-5, 2007 (PubMed).

Tsumura, Toshima, Leeksma et al.: "Sprouty-4 negatively regulates cell spreading by inhibiting the kinase activity of testicular protein kinase." in: The Biochemical journal, Vol. 387, Issue Pt 3, pp. 627-37, 2005 (PubMed).