TIE1 antibody (N-Term)

Catalog No. ABIN359947

Product Details anti-TIE1 Antibody

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects TIE1 at N-term.

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human TIE.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/10-1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TIE1

Target: TIE1 (tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 1 (TIE1))

Alternative Name: TIE1 (TIE1 Products)

Synonyms: JTK14 antibody, TIE antibody, D430008P04Rik antibody, tie-1 antibody, tyrosine kinase with immunoglobulin like and EGF like domains 1 antibody, tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody, tyrosine-protein kinase receptor Tie-1 antibody, endothelium-specific receptor tyrosine kinase 1 antibody, TIE1 antibody, Tie1 antibody, CpipJ_CPIJ018933 antibody, tie1 antibody

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).Synonyms: TIE, Tie-1, Tyrosine-protein kinase receptor Tie-1

Molecular Weight: 125089 Da

Gene ID: 7075, 9606

UniProt: P35590

Pathways: RTK Signaling