Angiotensin I Converting Enzyme 1 antibody

Catalog No. ABIN360872

The Rat Monoclonal anti-Angiotensin I Converting Enzyme 1 antibody is suitable to detect Angiotensin I Converting Enzyme 1 in samples from Mouse. It has been validated for WB.

Quick Overview for Angiotensin I Converting Enzyme 1 antibody (ABIN360872)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Angiotensin I Converting Enzyme 1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 9B21

Product Details anti-Angiotensin I Converting Enzyme 1 Antibody

Specificity: This antibody recognizes Mouse CD143. Other species not tested.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant Angiotensin-Converting Enzyme (ACE, CD143) protein.

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Buffer: 0.2 μm filtered PBS solution, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE / CD143

Background: Angiotensin-converting enzyme or ACE2, may also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE. Mouse ACE2 has about 40 % amino acid identity to the N-and C-terminal domains of mouse somatic ACE. The predicted mouse ACE 2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE 2 cleaves angiotensins I and II as a carboxypeptidase. ACE2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE2 activity. Novel peptide inhibitors of ACE2 do not inhibit ACE activity. Genetic data from Drosophila, mice and rats show that ACE2 is an essential regulator of heart function in vivo. In addition, ACE2 is a key SARSCoV Spike protein receptor in vivo and has a critical function in acute lung injury.Synonyms: Angiotensin-converting enzyme, DCP, DCP1, Dipeptidyl carboxypeptidase I

Gene ID: 11421

NCBI Accession: NP_033728

UniProt: P09470

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration