Angiotensin I Converting Enzyme 1 antibody

Catalog No. ABIN6939231

The Mouse Monoclonal anti-Angiotensin I Converting Enzyme 1 antibody is suitable to detect Angiotensin I Converting Enzyme 1 in samples from Human. It has been validated for ELISA.

Quick Overview for Angiotensin I Converting Enzyme 1 antibody (ABIN6939231)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Angiotensin I Converting Enzyme 1 antibody is un-conjugated

Application: ELISA

Clone: 9B9

Product Details anti-Angiotensin I Converting Enzyme 1 Antibody

Purpose: Angiotensin I Converting Enzyme (ACE) / CD143 Antibody

Specificity: This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Full-length recombinant human ACE/CD143 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Angiotensin I Converting Enzyme 1

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE

Background:

ACE, ACE1, angiotensin 1 converting enzyme 1, carboxycathepsin, CD143, CD143 antigen, DCP, DCP1, dipeptidyl carboxypeptidase 1, Kininase II, MVCD3, peptidase P, testicular ECA

Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate.

Molecular Weight: 150kDa

Gene ID: 1636, 298469

UniProt: P12821

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration