PCNA antibody
| Antigen |
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| Clonality |
Monoclonal (K1A3) |
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Host
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Reactivity
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Human (143), Rat (Rattus) (93), Mouse (Murine) (68), Cow (Bovine) (39), Dog (Canine) (39), Rabbit (36), Chicken (19), Horse (Equine) (6), Pig (Porcine) (5), Vertebrates (5), Fish (4), Insect (4), Yeast (Pichia pastoris) (4), Amphibian (3), Fruit Fly (Drosophila melanogaster) (3), Mammalian (3), Yeast (Saccharomyces Cerevisiae) (3), Monkey (1), Primate (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Application
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Western Blotting (WB) (104), Flow Cytometry (FACS) (69), Immunofluorescence (IF) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), Immunoprecipitation (IP) (31), ELISA (19), Immunohistochemistry (IHC) (17), Enzyme Immunoassay (EIA) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunocytochemistry (ICC) (5), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
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| Catalog no. |
ABIN361101 |
| Quantity |
50ul (1mg/ml) (Variants) |
| Price |
300.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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Recombinant human PCNA (1-261aa), Accession / GI number: NP_002583
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Isotype
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IgG3, kappa chain (Matching secondary antibodies)
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Clone
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K1A3
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Description
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PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors. Concentration: 1 mg/ml
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Application Notes
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Recommended dilution 1:2000
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Concentration
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1mg/ml
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Purification
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The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography
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Buffer
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In Phosphate-Buffered Saline (pH7.4) with 0.1% Sodium Azide
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Storage
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Store at -20°, Aliquot to avoid repeated freezing and thawing.
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Research Area
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Cell Cycle
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Restrictions
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For Research Use only
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Alternatives for antigen "PCNA", type "Antibodies"
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Hosts
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Mouse (101), Rabbit (82)
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Reactivities
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Human (143), Rat (Rattus) (93), Mouse (Murine) (68), Cow (Bovine) (39), Dog (Canine) (39), Rabbit (36), Chicken (19), Horse (Equine) (6), Pig (Porcine) (5), Vertebrates (5), Fish (4), Insect (4), Yeast (Pichia pastoris) (4), Amphibian (3), Fruit Fly (Drosophila melanogaster) (3), Mammalian (3), Yeast (Saccharomyces Cerevisiae) (3), Monkey (1), Primate (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (104), Flow Cytometry (FACS) (69), Immunofluorescence (IF) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), Immunoprecipitation (IP) (31), ELISA (19), Immunohistochemistry (IHC) (17), Enzyme Immunoassay (EIA) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunocytochemistry (ICC) (5), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2)
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Conjugates
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FITC (14), Biotin (10), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), PE (4), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), APC (1), RPE (1)
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Alternatives 
