Tryptophan Hydroxylase 1 antibody

Catalog No. ABIN361412

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for the ~55k tryptophan hydroxylase protein.

Cross-Reactivity: Human, Rat (Rattus)

Predicted Reactivity: wide variety mammalian

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, Haycock et al., 2002): 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: TPH1 (TPH1 Products)

Synonyms: TPRH antibody, TRPH antibody, tph antibody, tph1 antibody, tphD1 antibody, tph2 antibody, tph1l antibody, tphD2 antibody, Tph antibody, TPH antibody, SERGEF antibody, LOC100229598 antibody, tryptophan hydroxylase 1 antibody, tryptophan hydroxylase 1 (tryptophan 5-monooxygenase) a antibody, TryPtophan Hydroxylase antibody, tryptophan hydroxylase 1b antibody, tryptophan hydroxylase 1 L homeolog antibody, tryptophan 5-hydroxylase 1 antibody, TPH1 antibody, tph1a antibody, tph-1 antibody, tph1b antibody, Tph1 antibody, tph1.L antibody, LOAG_02149 antibody, Tsp_05203 antibody

Background: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001). Anti-Tryptophan Hydroxylase Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.

Molecular Weight: '55 kDa

Gene ID: 100101558

UniProt: P17290

Pathways: Feeding Behaviour