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Antibodies
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anti-RAF1 (BRAF) (pSer642) antibody
RAF1 (BRAF) (pSer642) antibody
| Antigen |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN361491 |
| Quantity |
100 µl |
| Price |
345.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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Phospho-Ser642 Raf-1
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Format
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Affinity-purified
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Description
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The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002, Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.
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Specificity
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Specific for the ~74k Raf-1 protein phosphorylated at Ser642.
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Application Notes
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Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.
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Purification
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Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and non-phosphopeptide affinity columns.
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Storage
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Store at -20 °C, stable for 1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "RAF1 (BRAF)", type "Antibodies"
Alternatives 
