Heat Shock Factor Protein 1 (HSF1) antibody

Antigen: Heat Shock Factor Protein 1 (HSF1)

Synonyms: ARABIDOPSIS THALIANA CLASS A HEAT SHOCK FACTOR 1A, ARABIDOPSIS THALIANA HEAT SHOCK FACTOR 1, ATHSF1, ATHSFA1A, CLASS A HEAT SHOCK FACTOR 1A, DL4910C, FCAALL.107, HEAT SHOCK FACTOR, HEAT SHOCK FACTOR 1, HSF1, HSFA1A, hsf1, Hsf, xhsf1, HSTF1, AA960185

Clonality: Monoclonal (10H8)

Host: Rat

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine), Guinea Pig, Hamster, Monkey, Rabbit

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), ELISA, Electrophoretic Mobility-Shift Assay (EMSA)

Catalog no.: ABIN361703

Additional Information

Characteristics: Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS PAGE immunoblots.

Alternative name: HSF1

Gene ID: 3297

Swiss-Prot: Q00613

Immunogen: Purified recombinant HSF1 protein

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 10H8

Description: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function . The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization. There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).
Synonyms: HSTF1

Specificity: Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots. Species cross-reactivity: Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit.

Sensitivity: 1 µg/mL of SMC-118 was sufficient for detection of HSF1 in 20µg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

Synonyms: ARABIDOPSIS THALIANA CLASS A HEAT SHOCK FACTOR 1A, ARABIDOPSIS THALIANA HEAT SHOCK FACTOR 1, ATHSF1, ATHSFA1A, CLASS A HEAT SHOCK FACTOR 1A, DL4910C, FCAALL.107, HEAT SHOCK FACTOR, HEAT SHOCK FACTOR 1, HSF1, HSFA1A, hsf1, Hsf, xhsf1, HSTF1, AA960185

Application Details

Concentration: 1 mg/mL

Purification: Protein G purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Heat Shock Proteins, Neurology

Restrictions: For Research Use only

Images

HSF1 (10H8), HeLa cells mitosis

Publications

Publications: McMillan, Xiao, Shao et al.: "Targeted disruption of heat shock transcription factor 1 abolishes thermotolerance and protection against heat-inducible apoptosis." in: The Journal of biological chemistry, Vol. 273, Issue 13, pp. 7523-8, 1998 (PubMed).

Morimoto: "Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators." in: Genes & development, Vol. 12, Issue 24, pp. 3788-96, 1999 (PubMed).

Jolly, Usson, Morimoto: "Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6769-74, 1999 (PubMed).

Morano, Thiele: "Heat shock factor function and regulation in response to cellular stress, growth, and differentiation signals." in: Gene expression, Vol. 7, Issue 4-6, pp. 271-82, 1999 (PubMed).

Tanaka, Namba, Arai et al.: "Genetic evidence for a protective role for heat shock factor 1 and heat shock protein 70 against colitis." in: The Journal of biological chemistry, Vol. 282, Issue 32, pp. 23240-52, 2007 (PubMed).

Monechi, Fiumalbi, De Monte et al.: "[Investigation on health status of silica exposed workers in "cotto Fiorentino" companies]" in: Giornale italiano di medicina del lavoro ed ergonomia, Vol. 29, Issue 3 Suppl, pp. 736-7, 2008 (PubMed).