RAB5 antibody (RAB5A, Member RAS Oncogene Family)

Details for Product anti-RAB5A Antibody No. ABIN361845, Supplier: Log in to see
Antigen
  • DDBDRAFT_0168594
  • DDBDRAFT_0229401
  • DDB_0168594
  • DDB_0229401
  • rab5
  • RAB5A
  • RAB5
  • 2410015H04Rik
  • AI663973
  • AU021172
  • nnyRab5a
  • rab5A protein
  • Rab5a, GTPase
  • hypothetical protein
  • Rab GTPase
  • Ras-related protein Rab-5A
  • ras-related protein rab-5a
  • RAB5A, member RAS oncogene family
  • rab5A
  • Rab5a
  • rab5a
  • RAB5A
  • LOC100350923
Alternatives
anti-Human RAB5 antibody for Immunofluorescence (fixed cells)
Reactivity
Cow (Bovine), Human, Monkey, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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44
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Clonality
Polyclonal
Conjugate
This RAB5 antibody is un-conjugated
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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11
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Options
Supplier
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Immunogen Human Rab5 synthetic peptide conjugated to KLH, identical to dog Rab5 sequence over the residues
Specificity Detects ~26 kDa.
Purification Protein A Purified
Alternative Name Rab5 (RAB5A Antibody Abstract)
Background Rab5 is a 24 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.
Gene ID 5868
NCBI Accession NP_004153
UniProt Q6FI44
Research Area Neurology, Organelles, Signaling
Pathways Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
Application Notes
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:80)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μl/ml of SPC-168 was sufficient for detection of Rab5 in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-RAB5 antibody (RAB5A, Member RAS Oncogene Family) (ABIN361845) Rab5, mouse backskin.
Western Blotting (WB) image for anti-RAB5 antibody (RAB5A, Member RAS Oncogene Family) (ABIN361845) WB 1 in 1000 Huma Cell line mix 20ug Rab5.
Product cited in: Ballmer-Hofer, Andersson, Ratcliffe, Berger: "Neuropilin-1 promotes VEGFR-2 trafficking through Rab11 vesicles thereby specifying signal output." in: Blood, Vol. 118, Issue 3, pp. 816-26, 2011 (PubMed).

Background publications Ali, Wasmeier, Lamoreux, Strom, Seabra: "Multiple regions contribute to membrane targeting of Rab GTPases." in: Journal of cell science, Vol. 117, Issue Pt 26, pp. 6401-12, 2004 (PubMed).

Stenmark, Olkkonen: "The Rab GTPase family." in: Genome biology, Vol. 2, Issue 5, pp. REVIEWS3007, 2001 (PubMed).

Takai, Sasaki, Matozaki: "Small GTP-binding proteins." in: Physiological reviews, Vol. 81, Issue 1, pp. 153-208, 2001 (PubMed).

Zerial, McBride: "Rab proteins as membrane organizers." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 2, pp. 107-17, 2001 (PubMed).

Woodman: "Biogenesis of the sorting endosome: the role of Rab5." in: Traffic (Copenhagen, Denmark), Vol. 1, Issue 9, pp. 695-701, 2001 (PubMed).

Sönnichsen, De Renzis, Nielsen, Rietdorf, Zerial: "Distinct membrane domains on endosomes in the recycling pathway visualized by multicolor imaging of Rab4, Rab5, and Rab11." in: The Journal of cell biology, Vol. 149, Issue 4, pp. 901-14, 2000 (PubMed).