FAK antibody (pTyr861)

Catalog No. ABIN361987

This Rabbit Polyclonal antibody specifically detects FAK in WB. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 4+ publications

Quick Overview for FAK antibody (pTyr861) (ABIN361987)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FAK Antibody

Binding Specificity: pTyr861

Specificity: The antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr861 (H-I-Y (p) -Q-P) derived from Human FAK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-FAK antibody (ABIN361987)

Kanehira, Kikuchi, Ohkouchi, Shibahara, Tode, Santoso, Daito, Ohta, Tamada, Nukiwa: "Targeting lysophosphatidic acid signaling retards culture-associated senescence of human marrow stromal cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e32185, (2012) (PubMed).

Sheng, Song, Zheng, Zhou, Lu, Zhao, Zhang, Gong: "Abnormal cleavage of APP impairs its functions in cell adhesion and migration." in: Neuroscience letters, Vol. 450, Issue 3, pp. 327-31, (2009) (PubMed).

Zheng, Wei, Wang, Gong, Zhang: "Surface characterization and cytocompatibility of three chitosan/polycation composite membranes for guided bone regeneration." in: Journal of biomaterials applications, Vol. 24, Issue 3, pp. 209-29, (2009) (PubMed).

Wu, Zhu, Shi, Zhang, Ma, Yu: "Zoledronate inhibits the proliferation, adhesion and migration of vascular smooth muscle cells." in: European journal of pharmacology, Vol. 602, Issue 1, pp. 124-31, (2008) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: FAK

Background: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Molecular Weight: 125 kDa

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling