FAK antibody (PTK2 Protein tyrosine Kinase 2) (pTyr576) Primary Antibody
- Target See all FAK (PTK2) Antibodies
- FAK (PTK2)
- Human, Mouse, Rat
- This FAK antibody is un-conjugated
- ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Phospho-FAK (Tyr576) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 576
- Human, Mouse (Murine), Rat (Rattus)
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- A synthesized peptide derived from human FAK around the phosphorylation site of Tyrosine 576
- Application Notes
- WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500
- For Research Use only
- 1 mg/mL
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- -20 °C
- Storage Comment
- Store at -20 °C.Stable for 12 months from date of receipt
- Expiry Date
- 12 months
- FAK (PTK2)
- Alternative Name
- FAK (PTK2 Products)
- FADK, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, PTK2, Fadk, mKIAA4203, fak, fak1a, ptk2, ptk2a, wu:fa18c04, zgc:66114, CG10023, CT28129, DFAK, DFak, DFak56, Dfak, Dfak56, DmFAK, Dmel\\CG10023, Fak56, Fak56D, fak56, protein tyrosine kinase 2, protein tyrosine kinase 2 L homeolog, PTK2 protein tyrosine kinase 2, protein tyrosine kinase 2ab, Focal adhesion kinase, PTK2, ptk2.L, Ptk2, ptk2ab, Fak
Description: Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation, required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation, this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC, this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1, most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1, GIT2 and SHC1, this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
- Molecular Weight
- Gene ID
- Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling