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Antibodies
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anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma...
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody
| Antigen |
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| Synonyms |
Neu, HER2, HER-2, c-neu, Erbb-2, c-erbB2, mKIAA3023, NEU, NGL, TKR1, CD340, HER-2/neu |
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Epitope
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pTyr1248 (16), pTyr877 (9), Ab-1221/1222 (5), pTyr1112 (5), pTyr1221/Tyr1222 (5), C-Term (4), pTyr1221/1222 (3), AA 1240-1260 (2), pSer1113 (2), pThr686 (2), AA 1243-1255 (1), AA 153-433 (1), N-Term (1), pTyr1139 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Western Blotting (WB) (142), Immunofluorescence (IF) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (67), Immunohistochemistry (IHC) (58), Enzyme Immunoassay (EIA) (35), Immunoprecipitation (IP) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (14), Flow Cytometry (FACS) (13), Functional Studies (Func) (4), Dot Blot (Dot) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN362015 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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HER2 (Phospho-Tyr877)
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Swiss-Prot
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P04626
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from humanHER2 around the phosphorylation site of tyrosine 877 (T-E-Y P -H-A).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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HER2 (Phospho-Tyr877) (Human) antibody detects endogenous levels of HER2 only whenphosphorylated at tyrosine 877.
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Application Notes
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WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2)", type "Antibodies"
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Hosts
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Rabbit (119), Mouse (58), Rat (4), Sheep (3), Chicken (2)
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Reactivities
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Human (172), Rat (Rattus) (72), Mouse (Murine) (69), Monkey (7), Dog (Canine) (6)
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Applications
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Western Blotting (WB) (142), Immunofluorescence (IF) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (67), Immunohistochemistry (IHC) (58), Enzyme Immunoassay (EIA) (35), Immunoprecipitation (IP) (20), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (14), Flow Cytometry (FACS) (13), Functional Studies (Func) (4), Dot Blot (Dot) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (3), FITC (1)
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Epitopes
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pTyr1248 (16), pTyr877 (9), Ab-1221/1222 (5), pTyr1112 (5), pTyr1221/Tyr1222 (5), C-Term (4), pTyr1221/1222 (3), AA 1240-1260 (2), pSer1113 (2), pThr686 (2), AA 1243-1255 (1), AA 153-433 (1), N-Term (1), pTyr1139 (1)
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