Retinoblastoma 1 antibody (pSer795)

Catalog No. ABIN362113

This Rabbit Polyclonal antibody specifically detects Retinoblastoma 1 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 2+ publications

Quick Overview for Retinoblastoma 1 antibody (pSer795) (ABIN362113)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: pSer795

Specificity: The antibody detects endogenous level of Rb only when phosphorylated at serine 795.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer795 (P-S-S (p) -P-L) derived from Human Rb. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Retinoblastoma 1 antibody (ABIN362113)

Braumüller, Wieder, Brenner, Aßmann, Hahn, Alkhaled, Schilbach, Essmann, Kneilling, Griessinger, Ranta, Ullrich, Mocikat, Braungart, Mehra, Fehrenbacher, Berdel, Niessner, Meier, van den Broek et al.: "T-helper-1-cell cytokines drive cancer into senescence. ..." in: Nature, Vol. 494, Issue 7437, pp. 361-5, (2013) (PubMed).

Sun, Fu, Liu, Tie, Zhu, Xing, Sun, Zheng: "Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest." in: FEBS letters, Vol. 582, Issue 10, pp. 1564-8, (2008) (PubMed).

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb

Background: Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Molecular Weight: 110 kDa

NCBI Accession: NP_000312

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy