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Retinoblastoma 1 (RB1) (pSer780) antibody

Antigen

Retinoblastoma 1 (RB1)

Synonyms RB, pRb, OSRC, pp110, p105-Rb, Rb, Rb-1, RB1, RB11, PP110, P105-RB
Epitope
Alternatives

pSer780

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Catalog no. ABIN362117
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name Rb (Phospho-Ser780)
Swiss-Prot P06400
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Rbaround the phosphorylation site of serine 780 (T-L-S P -P-I).
Isotype IgG  (Matching secondary antibodies)
Specificity Rb (Phospho-Ser780) (Human) antibody detects endogenous levels of Rb only when phosphorylatedat serine 780.

Application Details

Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Retinoblastoma 1 (RB1)", type "Antibodies"
Hosts Rabbit (114), Mouse (34), Rat (1)
Reactivities Human (139), Mouse (Murine) (74), Rat (Rattus) (72), Chicken (9), Dog (Canine) (1), Horse (Equine) (1), Mink (1)
Applications Western Blotting (WB) (121), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (54), Enzyme Immunoassay (EIA) (40), Immunohistochemistry (IHC) (40), Immunoprecipitation (IP) (20), Immunofluorescence (IF) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), ELISA (8), Dot Blot (Dot) (6), Flow Cytometry (FACS) (2)
Epitopes pSer807 (11), pSer780 (10), pSer795 (10), pSer612 (5), pSer608 (3), pSer811 (3), pThr356 (3), pThr826 (3), pSer249 (2), pSer788 (2), C-Term (1), S608 (1), S612 (1), S788 (1), pS249 (1), pS608 (1), pS780 (1), pS795 (1), pS811 (1), pSer249/pThr252 (1), pSer807/811 (1), pThr252 (1), pThr821 (1)