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Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody

Antigen

Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)
Synonyms KIP1, MEN4, CDKN4, MEN1B, P27KIP1, p27, Rpn4, MGC8644, Kip1, Cdki1b, sb:cb611, wu:fb63g10, wu:fb64g10, wu:fe18e03, CDKN1B
Epitope
Alternatives

pThr187
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Catalog no. ABIN362239
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name p27Kip1 (Phospho-Thr187)
Swiss-Prot P46527
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humanp27Kip1 around the phosphorylation site of threonine 187 (E-Q-T P -P-K).
Isotype IgG  (Matching secondary antibodies)
Specificity P27Kip1 (Phospho-Thr187) (Human) antibody detects endogenous levels of p27Kip1 only whenphosphorylated at threonine 187.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B)", type "Antibodies"
Hosts Rabbit (46), Mouse (16), Goat (6)
Reactivities Human (61), Mouse (Murine) (21), Rat (Rattus) (19), Dog (Canine) (4), Cat (Feline) (1), Cow (Bovine) (1), Monkey (1)
Applications Western Blotting (WB) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Enzyme Immunoassay (EIA) (15), Immunoprecipitation (IP) (11), ELISA (9), Immunohistochemistry (IHC) (8), Immunofluorescence (IF) (7), ELISA (Detection) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Dot Blot (Dot) (2)
Epitopes pThr187 (4), internal (2), pSer178 (2), pThr157 (2), pThr198 (2), C-Term (1), T198 (1), pS10 (1), pS178 (1), pSer10 (1), pT157 (1), pT187 (1)