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RPS6KB1 antibody (pThr421)

RPS6KB1 Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN362316
  • Target See all RPS6KB1 Antibodies
    RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
    Binding Specificity
    • 29
    • 28
    • 21
    • 19
    • 18
    • 16
    • 16
    • 16
    • 15
    • 15
    • 15
    • 15
    • 14
    • 13
    • 12
    • 11
    • 10
    • 8
    • 8
    • 8
    • 8
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr421
    Reactivity
    • 354
    • 260
    • 257
    • 57
    • 24
    • 23
    • 20
    • 19
    • 11
    • 9
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 334
    • 33
    • 4
    • 2
    Rabbit
    Clonality
    • 336
    • 37
    Polyclonal
    Conjugate
    • 171
    • 24
    • 17
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 6
    • 1
    • 1
    • 1
    This RPS6KB1 antibody is un-conjugated
    Application
    • 294
    • 147
    • 104
    • 94
    • 91
    • 77
    • 66
    • 32
    • 24
    • 24
    • 20
    • 8
    • 7
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Specificity
    The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at threonine 421.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around phosphorylation site of pThr421 (P-R-T (p) -P-V) derived from Human p70 S6 Kinase. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
    Top Product
    Discover our top product RPS6KB1 Primary Antibody
  • Application Notes
    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100
    Immunofluorescence: 1:100-1:200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Tang, Zhou, Zhou: "Dauricine inhibits insulin-like growth factor-I-induced hypoxia inducible factor 1alpha protein accumulation and vascular endothelial growth factor expression in human breast cancer cells." in: Acta pharmacologica Sinica, Vol. 30, Issue 5, pp. 605-16, (2009) (PubMed).

  • Target
    RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))
    Alternative Name
    p70 S6 Kinase (RPS6KB1 Products)
    Synonyms
    PS6K antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, STK14A antibody, p70 S6KA antibody, p70(S6K)-alpha antibody, p70-S6K antibody, p70-alpha antibody, 10539 antibody, 7084 antibody, CG10539 antibody, DS6K antibody, Dmel\\CG10539 antibody, Dp70S6k antibody, Dp70[s6k] antibody, Dp70s6k antibody, S6k antibody, dS6K antibody, dS6k antibody, dp70[S6k] antibody, dp70s6k antibody, dps6k antibody, ds6k antibody, fs(3)07084 antibody, l(3)07084 antibody, p70 S6K antibody, p70/S6K antibody, p70S6K antibody, p70[S6 kinase] antibody, p70[S6K] antibody, p70[S6k] antibody, p70[S6kinase] antibody, p70s6K antibody, s6k antibody, s6k11 antibody, 2610318I15Rik antibody, 4732464A07Rik antibody, 70kDa antibody, AA959758 antibody, AI256796 antibody, AI314060 antibody, p70/85s6k antibody, p70s6k antibody, p70s6k-A antibody, p70-s6k antibody, ps6k antibody, rps6kb1 antibody, rps6kb1-A antibody, s6K1 antibody, stk14a antibody, fc51h01 antibody, wu:fc51h01 antibody, zgc:55713 antibody, ribosomal protein S6 kinase B1 antibody, Ribosomal protein S6 kinase antibody, ribosomal protein S6 kinase, polypeptide 1 antibody, ribosomal protein S6 kinase B1 L homeolog antibody, ribosomal protein S6 kinase B1 S homeolog antibody, ribosomal protein S6 kinase b, polypeptide 1b antibody, RPS6KB1 antibody, S6k antibody, Rps6kb1 antibody, rps6kb1.L antibody, rps6kb1.S antibody, rps6kb1b antibody
    Background
    Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
    Molecular Weight
    85 kDa
    NCBI Accession
    NP_003152
    UniProt
    P23443
    Pathways
    PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect
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