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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody

Antigen

V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)

Synonyms
AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, ... show more
Epitope
Alternatives

pThr450

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Immunohistochemistry (IHC), Immunofluorescence (IF)
Catalog no. ABIN362435
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name AKT1 (phospho-Thr450)
Swiss-Prot P31749
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humanAKT1 around the phosphorylation site of threonine 450 (T-I-T P -P-P).
Isotype IgG  (Matching secondary antibodies)
Specificity AKT1 (phospho-Thr450) (Human) antibody detects endogenous levels of AKT1 only when phosphorylated at threonine 450.
Synonyms AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006

Application Details

Application Notes IHC: 1:50~100 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
Hosts Rabbit (162), Mouse (60), Sheep (4), Chicken (1)
Reactivities Human (214), Mouse (Murine) (121), Rat (Rattus) (112), Chicken (7), Dog (Canine) (4), Chimpanzee (2), Cow (Bovine) (2), Frog (1), Rabbit (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (183), Immunohistochemistry (IHC) (63), Enzyme Immunoassay (EIA) (60), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (59), ELISA (54), Immunofluorescence (IF) (44), Immunoprecipitation (IP) (16), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Dot Blot (Dot) (9), Flow Cytometry (FACS) (4), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
Conjugates Biotin (2), APC (1)
Epitopes pSer473 (21), pThr308 (14), pThr450 (4), pTyr315/316/312 (4), S129 (3), C-Term (2), N-Term (2), S246 (2), pS124 (2), pS473 (2), pThr308/309 (2), pY326 (2), N-Term G37 (1), S124 (1), S473 (1), pT308 (1)