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Aurora A antibody (Aurora Kinase A) (pThr288)

Details for Product anti-AURKA Antibody No. ABIN362453, Supplier: Log in to see
Antigen
Alternatives
anti-Human Aurora A antibody for Immunofluorescence
Epitope
pThr288
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5
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2
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1
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Reactivity
Human, Mouse (Murine)
218
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Host
Rabbit
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19
Clonality
Polyclonal
Conjugate
This Aurora A antibody is un-conjugated
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Application
Immunohistochemistry (IHC)
186
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77
50
34
28
15
15
12
5
4
2
1
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pThr288 (R-T-T (p) -L-M) derived from Human Aurora A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Aurora A (AURKA Antibody Abstract)
Background Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.
Molecular Weight 48 kDa
NCBI Accession NP_003591
UniProt O14965
Research Area Cancer, Cell Cycle
Pathways Cell Division Cycle
Application Notes Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Hauf, Cole, LaTerra et al.: "The small molecule Hesperadin reveals a role for Aurora B in correcting kinetochore-microtubule attachment and in maintaining the spindle assembly checkpoint." in: The Journal of cell biology, Vol. 161, Issue 2, pp. 281-94, 2003 (PubMed).

Walter, Seghezzi, Korver et al.: "The mitotic serine/threonine kinase Aurora2/AIK is regulated by phosphorylation and degradation." in: Oncogene, Vol. 19, Issue 42, pp. 4906-16, 2000 (PubMed).

Ellis: "The war of the sex chromosomes." in: Nature genetics, Vol. 20, Issue 1, pp. 9-10, 1998 (PubMed).

Bischoff, Anderson, Zhu et al.: "A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers." in: The EMBO journal, Vol. 17, Issue 11, pp. 3052-65, 1998 (PubMed).